amplican

This package is for version 3.6 of Bioconductor; for the stable, up-to-date release version, see amplican.

Automated analysis of CRISPR experiments.


Bioconductor version: 3.6

`amplican` performs alignment of the amplicon reads, normalizes gathered data, calculates multiple statistics (e.g. cut rates, frameshifts) and presents results in form of aggregated reports. Data and statistics can be broken down by experiments, barcodes, user defined groups, guides and amplicons allowing for quick identification of potential problems.

Author: Kornel Labun [aut], Eivind Valen [cph, cre]

Maintainer: Eivind Valen <eivind.valen at gmail.com>

Citation (from within R, enter citation("amplican")):

Installation

To install this package, start R (version "3.4") and enter:


if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("amplican")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

Reference Manual PDF
NEWS Text

Details

biocViews Alignment, CRISPR, Software, Technology, qPCR
Version 1.0.0
In Bioconductor since BioC 3.6 (R-3.4) (6.5 years)
License GPL-3
Depends R (>= 3.4.0), methods, BiocGenerics(>= 0.22.0), Biostrings(>= 2.44.2), data.table (>= 1.10.4)
Imports utils (>= 3.4.1), S4Vectors(>= 0.14.3), ShortRead(>= 1.34.0), IRanges(>= 2.10.2), GenomicRanges(>= 1.28.4), GenomeInfoDb(>= 1.12.2), BiocParallel(>= 1.10.1), gtable (>= 0.2.0), gridExtra (>= 2.2.1), ggplot2 (>= 2.2.0), ggbio(>= 1.24.1), ggthemes (>= 3.4.0), waffle (>= 0.7.0), stringr (>= 1.2.0), stats (>= 3.4.1), matrixStats (>= 0.52.2), Matrix (>= 1.2-10), dplyr (>= 0.7.2), rmarkdown (>= 1.6), knitr (>= 1.16), clusterCrit (>= 1.2.7)
System Requirements
URL https://github.com/valenlab/amplican
Bug Reports https://github.com/valenlab/amplican/issues
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Package Archives

Follow Installation instructions to use this package in your R session.

Source Package amplican_1.0.0.tar.gz
Windows Binary amplican_1.0.0.zip
Mac OS X 10.11 (El Capitan) amplican_1.0.0.tgz
Source Repository git clone https://git.bioconductor.org/packages/amplican
Source Repository (Developer Access) git clone git@git.bioconductor.org:packages/amplican
Package Short Url https://bioconductor.org/packages/amplican/
Package Downloads Report Download Stats
Old Source Packages for BioC 3.6 Source Archive